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16S rRNA RefSeq: V15.23    Genomic RefSeq: V10.1
Genome Explorer: Bradyrhizobium elkanii USDA 3259 (SEQF6046.1)
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PROKKA Annotation for SEQF6046.1
Genome selected: Bradyrhizobium elkanii USDA 3259
Contigs:101 Bases:8722461 bps
CDS:8137 rRNA:3
tmRNA:1 tRNA:58
Annotation Table [page:17]
Number of Records Found: 8137 [17 Pages]
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# Molecule PID NA (Length)(Seq)
AA (Length)(Seq)
Range Gene Gene Product
8001 SEQF6046.1_AXAW01000089.1 SEQF6046.1_07962 JB 600 [NA] 199 [AA] 915-1514 sodB Superoxide dismutase [Mn]
8002 SEQF6046.1_AXAW01000089.1 SEQF6046.1_07963 JB 531 [NA] 176 [AA] 1654-2184 hypothetical protein
8003 SEQF6046.1_AXAW01000089.1 SEQF6046.1_07964 JB 1380 [NA] 459 [AA] 2321-3700 hypothetical protein
8004 SEQF6046.1_AXAW01000090.1 SEQF6046.1_07965 JB 774 [NA] 257 [AA] 49-822 hypothetical protein
8005 SEQF6046.1_AXAW01000090.1 SEQF6046.1_07966 JB 1233 [NA] 410 [AA] 1087-2319 hypothetical protein
8006 SEQF6046.1_AXAW01000090.1 SEQF6046.1_07967 JB 1284 [NA] 427 [AA] 2342-3625 ttuD_2 Putative hydroxypyruvate reductase
8007 SEQF6046.1_AXAW01000091.1 SEQF6046.1_07968 JB 261 [NA] 86 [AA] 774-1034 hypothetical protein
8008 SEQF6046.1_AXAW01000091.1 SEQF6046.1_07969 JB 1026 [NA] 341 [AA] 1034-2059 IS4 family transposase ISPye60
8009 SEQF6046.1_AXAW01000092.1 SEQF6046.1_08232 JB 657 [NA] 218 [AA] 207-863 hypothetical protein
8010 SEQF6046.1_AXAW01000092.1 SEQF6046.1_08233 JB 888 [NA] 295 [AA] 1480-2367 IS66 family transposase ISRm2
8011 SEQF6046.1_AXAW01000093.1 SEQF6046.1_08235 JB 1335 [NA] 444 [AA] 599-1933 hypothetical protein
8012 SEQF6046.1_AXAW01000094.1 SEQF6046.1_08236 JB 336 [NA] 111 [AA] 46-381 hypothetical protein
8013 SEQF6046.1_AXAW01000094.1 SEQF6046.1_08237 JB 336 [NA] 111 [AA] 1162-1497 hypothetical protein
8014 SEQF6046.1_AXAW01000095.1 SEQF6046.1_08238 JB 675 [NA] 224 [AA] 686-1360 hypothetical protein
8015 SEQF6046.1_AXAW01000095.1 SEQF6046.1_08239 JB 345 [NA] 114 [AA] 1432-1776 hypothetical protein
8016 SEQF6046.1_AXAW01000096.1 SEQF6046.1_08240 JB 207 [NA] 68 [AA] 39-245 hypothetical protein
8017 SEQF6046.1_AXAW01000096.1 SEQF6046.1_08241 JB 474 [NA] 157 [AA] 394-867 IS3 family transposase ISRj2
8018 SEQF6046.1_AXAW01000096.1 SEQF6046.1_08242 JB 306 [NA] 101 [AA] 1216-1521 hypothetical protein
8019 SEQF6046.1_AXAW01000097.1 SEQF6046.1_08243 JB 195 [NA] 64 [AA] 38-232 hypothetical protein
8020 SEQF6046.1_AXAW01000097.1 SEQF6046.1_08244 JB 774 [NA] 257 [AA] 653-1426 hypothetical protein
8021 SEQF6046.1_AXAW01000098.1 SEQF6046.1_08245 JB 588 [NA] 195 [AA] 227-814 hypothetical protein
8022 SEQF6046.1_AXAW01000099.1 SEQF6046.1_08246 JB 510 [NA] 169 [AA] 353-862 IS66 family transposase ISRm2
8023 SEQF6046.1_AXAW01000099.1 SEQF6046.1_08247 JB 255 [NA] 84 [AA] 915-1169 IS66 family transposase IS1313
8024 SEQF6046.1_AXAW01000100.1 SEQF6046.1_08248 JB 348 [NA] 115 [AA] 451-798 hypothetical protein
8025 SEQF6046.1_AXAW01000100.1 SEQF6046.1_08249 JB 399 [NA] 132 [AA] 795-1193 hypothetical protein
8026 SEQF6046.1_KI421486.1 SEQF6046.1_02527 JB 321 [NA] 106 [AA] 287-607 fdxE Ferredoxin-6
8027 SEQF6046.1_KI421486.1 SEQF6046.1_02528 JB 378 [NA] 125 [AA] 769-1146 hypothetical protein
8028 SEQF6046.1_KI421486.1 SEQF6046.1_02529 JB 624 [NA] 207 [AA] 1318-1941 hypothetical protein
8029 SEQF6046.1_KI421486.1 SEQF6046.1_02530 JB 912 [NA] 303 [AA] 2239-3150 hypothetical protein
8030 SEQF6046.1_KI421486.1 SEQF6046.1_02531 JB 444 [NA] 147 [AA] 3169-3612 hypothetical protein
8031 SEQF6046.1_KI421486.1 SEQF6046.1_02532 JB 879 [NA] 292 [AA] 3685-4563 hypothetical protein
8032 SEQF6046.1_KI421486.1 SEQF6046.1_02533 JB 2679 [NA] 892 [AA] 4847-7525 hypothetical protein
8033 SEQF6046.1_KI421486.1 SEQF6046.1_02534 JB 867 [NA] 288 [AA] 7754-8620 hypothetical protein
8034 SEQF6046.1_KI421486.1 SEQF6046.1_02535 JB 771 [NA] 256 [AA] 8739-9509 mkl_1 putative ribonucleotide transport ATP-binding protein mkl
8035 SEQF6046.1_KI421486.1 SEQF6046.1_02536 JB 1134 [NA] 377 [AA] 9512-10645 hypothetical protein
8036 SEQF6046.1_KI421486.1 SEQF6046.1_02537 JB 1002 [NA] 333 [AA] 10787-11788 ycjG L-Ala-DL-Glu epimerase
8037 SEQF6046.1_KI421486.1 SEQF6046.1_02538 JB 1173 [NA] 390 [AA] 11785-12957 hcaT putative 3-phenylpropionic acid transporter
8038 SEQF6046.1_KI421486.1 SEQF6046.1_02539 JB 1278 [NA] 425 [AA] 13033-14310 hypothetical protein
8039 SEQF6046.1_KI421486.1 SEQF6046.1_02540 JB 990 [NA] 329 [AA] 14600-15589 thadh L-threo-3-hydroxyaspartate ammonia-lyase
8040 SEQF6046.1_KI421486.1 SEQF6046.1_02541 JB 1092 [NA] 363 [AA] 15646-16737 mshA_6 D-inositol-3-phosphate glycosyltransferase
8041 SEQF6046.1_KI421486.1 SEQF6046.1_02542 JB 813 [NA] 270 [AA] 16734-17546 lpxH UDP-2%2C3-diacylglucosamine hydrolase
8042 SEQF6046.1_KI421486.1 SEQF6046.1_02543 JB 798 [NA] 265 [AA] 17950-18747 thiD Hydroxymethylpyrimidinephosphomethylpyrimidine kinase
8043 SEQF6046.1_KI421486.1 SEQF6046.1_02544 JB 246 [NA] 81 [AA] 18943-19188 hypothetical protein
8044 SEQF6046.1_KI421486.1 SEQF6046.1_02545 JB 930 [NA] 309 [AA] 19311-20240 nicB_3 Nicotinate dehydrogenase subunit B
8045 SEQF6046.1_KI421486.1 SEQF6046.1_02546 JB 423 [NA] 140 [AA] 20406-20828 hypothetical protein
8046 SEQF6046.1_KI421486.1 SEQF6046.1_02547 JB 447 [NA] 148 [AA] 20978-21424 Cytochrome c-556
8047 SEQF6046.1_KI421486.1 SEQF6046.1_02548 JB 2310 [NA] 769 [AA] 21529-23838 cutL_3 Carbon monoxide dehydrogenase large chain
8048 SEQF6046.1_KI421486.1 SEQF6046.1_02549 JB 1530 [NA] 509 [AA] 23967-25496 murJ_2 putative lipid II flippase MurJ
8049 SEQF6046.1_KI421486.1 SEQF6046.1_02550 JB 1152 [NA] 383 [AA] 25540-26691 wbpE UDP-2-acetamido-2-deoxy-3-oxo-D-glucuronate aminotransferase
8050 SEQF6046.1_KI421486.1 SEQF6046.1_02551 JB 1017 [NA] 338 [AA] 26723-27739 iolX scyllo-inositol 2-dehydrogenase (NAD(+))
8051 SEQF6046.1_KI421486.1 SEQF6046.1_02552 JB 1413 [NA] 470 [AA] 27991-29403 algA Alginate biosynthesis protein AlgA
8052 SEQF6046.1_KI421486.1 SEQF6046.1_02553 JB 987 [NA] 328 [AA] 29756-30742 hypothetical protein
8053 SEQF6046.1_KI421486.1 SEQF6046.1_02554 JB 1011 [NA] 336 [AA] 30753-31763 tagO putative undecaprenyl-phosphate N-acetylglucosaminyl 1-phosphate transferase
8054 SEQF6046.1_KI421486.1 SEQF6046.1_02555 JB 942 [NA] 313 [AA] 31769-32710 gnu N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans%2Coctacis-undecaprenol 4-epimerase
8055 SEQF6046.1_KI421486.1 SEQF6046.1_02556 JB 1215 [NA] 404 [AA] 32707-33921 hypothetical protein
8056 SEQF6046.1_KI421486.1 SEQF6046.1_02557 JB 1914 [NA] 637 [AA] 33970-35883 pglF UDP-N-acetyl-alpha-D-glucosamine C6 dehydratase
8057 SEQF6046.1_KI421486.1 SEQF6046.1_02558 JB 1281 [NA] 426 [AA] 35969-37249 hypothetical protein
8058 SEQF6046.1_KI421486.1 SEQF6046.1_02559 JB 1473 [NA] 490 [AA] 37258-38730 hldE Bifunctional protein HldE
8059 SEQF6046.1_KI421486.1 SEQF6046.1_02560 JB 981 [NA] 326 [AA] 38748-39728 hldD ADP-L-glycero-D-manno-heptose-6-epimerase
8060 SEQF6046.1_KI421486.1 SEQF6046.1_02561 JB 1065 [NA] 354 [AA] 39864-40928 rfaF ADP-heptose--LPS heptosyltransferase 2
8061 SEQF6046.1_KI421486.1 SEQF6046.1_02562 JB 1047 [NA] 348 [AA] 40910-41956 mshA_7 D-inositol-3-phosphate glycosyltransferase
8062 SEQF6046.1_KI421486.1 SEQF6046.1_02563 JB 1014 [NA] 337 [AA] 42089-43102 galE_4 UDP-glucose 4-epimerase
8063 SEQF6046.1_KI421486.1 SEQF6046.1_02564 JB 1812 [NA] 603 [AA] 43212-45023 msbA_1 Lipid A export ATP-bindingpermease protein MsbA
8064 SEQF6046.1_KI421486.1 SEQF6046.1_02565 JB 1179 [NA] 392 [AA] 45030-46208 hypothetical protein
8065 SEQF6046.1_KI421486.1 SEQF6046.1_02566 JB 702 [NA] 233 [AA] 46362-47063 hypothetical protein
8066 SEQF6046.1_KI421486.1 SEQF6046.1_02567 JB 225 [NA] 74 [AA] 47132-47356 hypothetical protein
8067 SEQF6046.1_KI421486.1 SEQF6046.1_02568 JB 273 [NA] 90 [AA] 47565-47837 hypothetical protein
8068 SEQF6046.1_KI421486.1 SEQF6046.1_02569 JB 429 [NA] 142 [AA] 47927-48355 hypothetical protein
8069 SEQF6046.1_KI421486.1 SEQF6046.1_02570 JB 1365 [NA] 454 [AA] 48324-49688 abaF_1 Fosfomycin resistance protein AbaF
8070 SEQF6046.1_KI421486.1 SEQF6046.1_02571 JB 1683 [NA] 560 [AA] 49958-51640 ywrD_3 Glutathione hydrolase-like YwrD proenzyme
8071 SEQF6046.1_KI421486.1 SEQF6046.1_02572 JB 1698 [NA] 565 [AA] 51894-53591 maeA NAD-dependent malic enzyme
8072 SEQF6046.1_KI421486.1 SEQF6046.1_02573 JB 156 [NA] 51 [AA] 53917-54072 hypothetical protein
8073 SEQF6046.1_KI421486.1 SEQF6046.1_02574 JB 930 [NA] 309 [AA] 54163-55092 hypothetical protein
8074 SEQF6046.1_KI421486.1 SEQF6046.1_02575 JB 1200 [NA] 399 [AA] 55448-56647 hypothetical protein
8075 SEQF6046.1_KI421486.1 SEQF6046.1_02576 JB 1719 [NA] 572 [AA] 56658-58376 kstD 3-oxosteroid 1-dehydrogenase
8076 SEQF6046.1_KI421486.1 SEQF6046.1_02577 JB 765 [NA] 254 [AA] 58373-59137 idnO_3 5-keto-D-gluconate 5-reductase
8077 SEQF6046.1_KI421486.1 SEQF6046.1_02578 JB 801 [NA] 266 [AA] 59165-59965 gylR Glycerol operon regulatory protein
8078 SEQF6046.1_KI421486.1 SEQF6046.1_02579 JB 1062 [NA] 353 [AA] 60551-61612 hypothetical protein
8079 SEQF6046.1_KI421486.1 SEQF6046.1_02580 JB 1428 [NA] 475 [AA] 61936-63363 ahcY Adenosylhomocysteinase
8080 SEQF6046.1_KI421486.1 SEQF6046.1_02581 JB 1197 [NA] 398 [AA] 63490-64686 metK S-adenosylmethionine synthase
8081 SEQF6046.1_KI421486.1 SEQF6046.1_02582 JB 1356 [NA] 451 [AA] 64747-66102 hypothetical protein
8082 SEQF6046.1_KI421486.1 SEQF6046.1_02583 JB 843 [NA] 280 [AA] 66451-67293 hypothetical protein
8083 SEQF6046.1_KI421486.1 SEQF6046.1_02584 JB 1488 [NA] 495 [AA] 67314-68801 arnC_1 Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase
8084 SEQF6046.1_KI421486.1 SEQF6046.1_02585 JB 750 [NA] 249 [AA] 68812-69561 hypothetical protein
8085 SEQF6046.1_KI421486.1 SEQF6046.1_02586 JB 2601 [NA] 866 [AA] 69563-72163 hypothetical protein
8086 SEQF6046.1_KI421486.1 SEQF6046.1_02587 JB 1728 [NA] 575 [AA] 72387-74114 ycf3_1 Photosystem I assembly protein Ycf3
8087 SEQF6046.1_KI421486.1 SEQF6046.1_02588 JB 1119 [NA] 372 [AA] 74128-75246 hypothetical protein
8088 SEQF6046.1_KI421486.1 SEQF6046.1_02589 JB 717 [NA] 238 [AA] 75278-75994 livF_10 High-affinity branched-chain amino acid transport ATP-binding protein LivF
8089 SEQF6046.1_KI421486.1 SEQF6046.1_02590 JB 753 [NA] 250 [AA] 75987-76739 lptB_7 Lipopolysaccharide export system ATP-binding protein LptB
8090 SEQF6046.1_KI421486.1 SEQF6046.1_02591 JB 1032 [NA] 343 [AA] 76736-77767 hypothetical protein
8091 SEQF6046.1_KI421486.1 SEQF6046.1_02592 JB 870 [NA] 289 [AA] 77775-78644 livH_10 High-affinity branched-chain amino acid transport system permease protein LivH
8092 SEQF6046.1_KI421486.1 SEQF6046.1_02593 JB 1182 [NA] 393 [AA] 78803-79984 braC_3 Leucine-%2C isoleucine-%2C valine-%2C threonine-%2C and alanine-binding protein
8093 SEQF6046.1_KI421486.1 SEQF6046.1_02594 JB 363 [NA] 120 [AA] 80277-80639 hypothetical protein
8094 SEQF6046.1_KI421486.1 SEQF6046.1_02595 JB 864 [NA] 287 [AA] 81536-82399 purU Formyltetrahydrofolate deformylase
8095 SEQF6046.1_KI421486.1 SEQF6046.1_02596 JB 846 [NA] 281 [AA] 82536-83381 hypothetical protein
8096 SEQF6046.1_KI421486.1 SEQF6046.1_02597 JB 978 [NA] 325 [AA] 83480-84457 hypothetical protein
8097 SEQF6046.1_KI421486.1 SEQF6046.1_02598 JB 945 [NA] 314 [AA] 84575-85519 fcl_1 GDP-L-fucose synthase
8098 SEQF6046.1_KI421486.1 SEQF6046.1_02599 JB 1101 [NA] 366 [AA] 85500-86600 gmd_1 GDP-mannose 4%2C6-dehydratase
8099 SEQF6046.1_KI421486.1 SEQF6046.1_02600 JB 675 [NA] 224 [AA] 86738-87412 Cyclic AMP receptor protein
8100 SEQF6046.1_KI421486.1 SEQF6046.1_02601 JB 822 [NA] 273 [AA] 87800-88621 lptB_8 Lipopolysaccharide export system ATP-binding protein LptB
8101 SEQF6046.1_KI421486.1 SEQF6046.1_02602 JB 1929 [NA] 642 [AA] 88618-90546 Long-chain-fatty-acid--CoA ligase FadD15
8102 SEQF6046.1_KI421486.1 SEQF6046.1_02603 JB 225 [NA] 74 [AA] 90543-90767 hypothetical protein
8103 SEQF6046.1_KI421486.1 SEQF6046.1_02604 JB 894 [NA] 297 [AA] 90764-91657 livH_11 High-affinity branched-chain amino acid transport system permease protein LivH
8104 SEQF6046.1_KI421486.1 SEQF6046.1_02605 JB 1074 [NA] 357 [AA] 91661-92734 hypothetical protein
8105 SEQF6046.1_KI421486.1 SEQF6046.1_02606 JB 1284 [NA] 427 [AA] 92796-94079 hypothetical protein
8106 SEQF6046.1_KI421486.1 SEQF6046.1_02607 JB 840 [NA] 279 [AA] 94210-95049 livF_11 High-affinity branched-chain amino acid transport ATP-binding protein LivF
8107 SEQF6046.1_KI421486.1 SEQF6046.1_02608 JB 1152 [NA] 383 [AA] 95114-96265 ce Cellobiose 2-epimerase
8108 SEQF6046.1_KI421486.1 SEQF6046.1_02609 JB 1224 [NA] 407 [AA] 96361-97584 Phenylacetate-coenzyme A ligase
8109 SEQF6046.1_KI421486.1 SEQF6046.1_02610 JB 531 [NA] 176 [AA] 97689-98219 gmhB D-glycero-beta-D-manno-heptose-1%2C7-bisphosphate 7-phosphatase
8110 SEQF6046.1_KI421486.1 SEQF6046.1_02611 JB 1098 [NA] 365 [AA] 98257-99354 desV dTDP-3-amino-3%2C4%2C6-trideoxy-alpha-D-glucose transaminase
8111 SEQF6046.1_KI421486.1 SEQF6046.1_02612 JB 468 [NA] 155 [AA] 99373-99840 lpxD_1 UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
8112 SEQF6046.1_KI421486.1 SEQF6046.1_02613 JB 1062 [NA] 353 [AA] 99837-100898 iolG_2 Inositol 2-dehydrogenaseD-chiro-inositol 3-dehydrogenase
8113 SEQF6046.1_KI421486.1 SEQF6046.1_02614 JB 1017 [NA] 338 [AA] 101128-102144 galE1_2 UDP-glucose 4-epimerase
8114 SEQF6046.1_KI421486.1 SEQF6046.1_02615 JB 1290 [NA] 429 [AA] 102164-103453 wbpA UDP-N-acetyl-D-glucosamine 6-dehydrogenase
8115 SEQF6046.1_KI421486.1 SEQF6046.1_02616 JB 1935 [NA] 644 [AA] 103896-105830 asnB_2 Asparagine synthetase [glutamine-hydrolyzing] 1
8116 SEQF6046.1_KI421486.1 SEQF6046.1_02617 JB 834 [NA] 277 [AA] 105832-106665 bshA N-acetyl-alpha-D-glucosaminyl L-malate synthase
8117 SEQF6046.1_KI421486.1 SEQF6046.1_02618 JB 486 [NA] 161 [AA] 106735-107220 hypothetical protein
8118 SEQF6046.1_KI421486.1 SEQF6046.1_02619 JB 810 [NA] 269 [AA] 107115-107924 COQ3_3 Ubiquinone biosynthesis O-methyltransferase%2C mitochondrial
8119 SEQF6046.1_KI421486.1 SEQF6046.1_02620 JB 1317 [NA] 438 [AA] 107947-109263 mshA_8 D-inositol-3-phosphate glycosyltransferase
8120 SEQF6046.1_KI421486.1 SEQF6046.1_02621 JB 2166 [NA] 721 [AA] 109740-111905 mshA_9 D-inositol-3-phosphate glycosyltransferase
8121 SEQF6046.1_KI421486.1 SEQF6046.1_02622 JB 879 [NA] 292 [AA] 111949-112827 hypothetical protein
8122 SEQF6046.1_KI421486.1 SEQF6046.1_02623 JB 1344 [NA] 447 [AA] 112821-114164 btuD_7 Vitamin B12 import ATP-binding protein BtuD
8123 SEQF6046.1_KI421486.1 SEQF6046.1_02624 JB 801 [NA] 266 [AA] 114161-114961 hypothetical protein
8124 SEQF6046.1_KI421486.1 SEQF6046.1_02625 JB 1944 [NA] 647 [AA] 114958-116901 asnB_3 Asparagine synthetase [glutamine-hydrolyzing] 1
8125 SEQF6046.1_KI421486.1 SEQF6046.1_02626 JB 642 [NA] 213 [AA] 116986-117627 hypothetical protein
8126 SEQF6046.1_KI421486.1 SEQF6046.1_02627 JB 1185 [NA] 394 [AA] 118346-119530 pglA N%2CN-diacetylbacillosaminyl-diphospho-undecaprenol alpha-1%2C3-N-acetylgalactosaminyltransferase
8127 SEQF6046.1_KI421486.1 SEQF6046.1_02628 JB 1332 [NA] 443 [AA] 120235-121566 hypothetical protein
8128 SEQF6046.1_KI421486.1 SEQF6046.1_02629 JB 258 [NA] 85 [AA] 121723-121980 hypothetical protein
8129 SEQF6046.1_KI421486.1 SEQF6046.1_02630 JB 990 [NA] 329 [AA] 122051-123040 hypothetical protein
8130 SEQF6046.1_KI421486.1 SEQF6046.1_02631 JB 1161 [NA] 386 [AA] 123474-124634 hypothetical protein
8131 SEQF6046.1_KI421486.1 SEQF6046.1_02632 JB 930 [NA] 309 [AA] 124631-125560 rmd GDP-6-deoxy-D-mannose reductase
8132 SEQF6046.1_KI421486.1 SEQF6046.1_02633 JB 564 [NA] 187 [AA] 125579-126142 dapH_2 2%2C3%2C4%2C5-tetrahydropyridine-2%2C6-dicarboxylate N-acetyltransferase
8133 SEQF6046.1_KI421486.1 SEQF6046.1_02634 JB 855 [NA] 284 [AA] 126139-126993 hypothetical protein
8134 SEQF6046.1_KI421486.1 SEQF6046.1_02635 JB 1089 [NA] 362 [AA] 127002-128090 hypothetical protein
8135 SEQF6046.1_KI421486.1 SEQF6046.1_02636 JB 1839 [NA] 612 [AA] 128094-129932 asnB_4 Asparagine synthetase [glutamine-hydrolyzing] 1
8136 SEQF6046.1_KI421486.1 SEQF6046.1_02637 JB 1554 [NA] 517 [AA] 129935-131488 murJ_3 lipid II flippase MurJ
8137 SEQF6046.1_KI421486.1 SEQF6046.1_02638 JB 225 [NA] 74 [AA] 132424-132648 IS481 family transposase ISRel12